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Addgene

pRSF-coSnaA-TEV-His_His-TEV-SnaC
(Plasmid #225059)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225059 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSFDuet
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SnaA
  • Species
    Stackebrandtia nassauensis
  • Insert Size (bp)
    5070
  • Promoter T7

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

also encodes SnaC

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSF-coSnaA-TEV-His_His-TEV-SnaC was a gift from Wilfred van der Donk (Addgene plasmid # 225059 ; http://n2t.net/addgene:225059 ; RRID:Addgene_225059)

  • For your References section:

    PEARL-Catalyzed Peptide Bond Formation after Chain Reversal by Ureido-Forming Condensation Domains. Yu Y, van der Donk WA. ACS Cent Sci. 2024 Jun 3;10(6):1242-1250. doi: 10.1021/acscentsci.4c00044. eCollection 2024 Jun 26. 10.1021/acscentsci.4c00044 PubMed 38947204
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