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Addgene

POSV801-SP44-SnaABCDEOinT1T2
(Plasmid #225058)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225058 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pOSV801
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Apramycin, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    inactivated SnaO
  • Species
    Stackebrandtia nassauensis
  • Insert Size (bp)
    279
  • Mutation
    deltaR51-V511
  • Promoter SP44

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

also encodes SnaA, SnaB, SnaC, SnaD, SnaE, SnaT1, SnaT2

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    POSV801-SP44-SnaABCDEOinT1T2 was a gift from Wilfred van der Donk (Addgene plasmid # 225058 ; http://n2t.net/addgene:225058 ; RRID:Addgene_225058)
  • For your References section:

    PEARL-Catalyzed Peptide Bond Formation after Chain Reversal by Ureido-Forming Condensation Domains. Yu Y, van der Donk WA. ACS Cent Sci. 2024 Jun 3;10(6):1242-1250. doi: 10.1021/acscentsci.4c00044. eCollection 2024 Jun 26. 10.1021/acscentsci.4c00044 PubMed 38947204