POSV801-SP44-SnaABCDEOinT1T2
(Plasmid
#225058)
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PurposeHeterologous expression in Streptomyces with inactive SnaO
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 225058 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepOSV801
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Apramycin, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameinactivated SnaO
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SpeciesStackebrandtia nassauensis
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Insert Size (bp)279
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MutationdeltaR51-V511
- Promoter SP44
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
also encodes SnaA, SnaB, SnaC, SnaD, SnaE, SnaT1, SnaT2
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
POSV801-SP44-SnaABCDEOinT1T2 was a gift from Wilfred van der Donk (Addgene plasmid # 225058 ; http://n2t.net/addgene:225058 ; RRID:Addgene_225058) -
For your References section:
PEARL-Catalyzed Peptide Bond Formation after Chain Reversal by Ureido-Forming Condensation Domains. Yu Y, van der Donk WA. ACS Cent Sci. 2024 Jun 3;10(6):1242-1250. doi: 10.1021/acscentsci.4c00044. eCollection 2024 Jun 26. 10.1021/acscentsci.4c00044 PubMed 38947204