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pLV-mRFP1-Human UTROPHIN_1-261
(Plasmid #225052)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225052 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti-MP2
  • Backbone manufacturer
    Pantelis Tsoulfas
  • Backbone size w/o insert (bp) 6698
  • Total vector size (bp) 8166
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Human Utrophin residues 1-261 and RFP1
  • Alt name
    dystrophin-related protein
  • Species
    H. sapiens (human); Homo Sapiens
  • Insert Size (bp)
    1470
  • Mutation
    Sequence is derived from ADDGENE Plasmid #26739-RFP
  • GenBank ID
    X69086
  • Entrez Gene
    UTRN (a.k.a. DMDL, DRP, DRP1)
  • Promoter CMV promoter and enhancer
  • Tags / Fusion Proteins
    • mRFP1 (N terminal on insert)
    • Human UTROPHIN 1-261 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site None (destroyed during cloning)
  • 5′ sequencing primer LNCX
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-mRFP1-Human UTROPHIN_1-261 was a gift from Pantelis Tsoulfas (Addgene plasmid # 225052 ; http://n2t.net/addgene:225052 ; RRID:Addgene_225052)
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