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Addgene

Ubc.IkBSR-Flag Pgk.Cre
(Plasmid #22503)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22503 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    UbC.Luciferase.PGK.Cre
  • Backbone manufacturer
    Available at Addgene (#20905)
  • Backbone size w/o insert (bp) 8609
  • Vector type
    Mammalian Expression, Lentiviral, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    IkBSR
  • Alt name
    IkappaBalpha super repressor
  • Alt name
    IkB-SR
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    969
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer MSCV rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mouse IkappaBalpha super repressor, Flag tagged (C-terminus). Cloned EcoRI-NotI in UbC.Luciferase.PGK.Cre vector in place of Luciferase: UBC promoter-Kozak-IkBaSR-Flag-STOP—PGK promoter-Cre.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ubc.IkBSR-Flag Pgk.Cre was a gift from Tyler Jacks (Addgene plasmid # 22503 ; http://n2t.net/addgene:22503 ; RRID:Addgene_22503)

  • For your References section:

    Requirement for NF-kappaB signalling in a mouse model of lung adenocarcinoma. Meylan E, Dooley AL, Feldser DM, Shen L, Turk E, Ouyang C, Jacks T. Nature. 2009 Nov 5;462(7269):104-7. doi: 10.1038/nature08462. Epub 2009 Oct 21. 10.1038/nature08462 PubMed 19847165
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