Ubc.IkBSR-Flag Pgk.Cre
(Plasmid #22503)

• Depositing Lab: Tyler Jacks
• Publication: Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi: 10.1038/nature08462. Epub 2009 Oct 21.

Backbone

• Vector backbone: UbC.Luciferase.PGK.Cre
• Backbone manufacturer: Available at Addgene (#20905)
• Backbone size w/o insert (bp): 8609
• Vector type: Mammalian Expression, Lentiviral, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: IkBSR
• Alt name: IkappaBalpha super repressor
• Alt name: IkB-SR
• Species: M. musculus (mouse)
• Insert Size (bp): 969
• Tag / Fusion Protein:
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: MSCV rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Mouse IkappaBalpha super repressor, Flag tagged (C-terminus). Cloned EcoRI-NotI in UbC.Luciferase.PGK.Cre vector in place of Luciferase: UBC promoter-Kozak-IkBaSR-Flag-STOP—PGK promoter-Cre.