pCEP4-tat
(Plasmid #22502)

• Depositing Lab: Sergey Kasparov
• Publication: Coleman et al Physiol Genomics. 2003 Feb 6. 12(3):221-8.

Backbone

• Vector backbone: pCEP4
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 10000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Transform into DH5alpha and grow in Luria Broth containing 100µg/ml ampicillin.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Transactivator of Transcription
• Species: Retrovirus

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: N/A

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Original plasmid sent by Jason Coleman and Matthew Huentelman.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid forms part of a plasmid set for lentivirus generation in the Kasparov lab, University of Bristol, UK.

Diagnostic digest: Digest with HindIII, expect 0.25 and 10 kb fragments.