pNHP
(Plasmid #22500)

• Depositing Lab: Sergey Kasparov
• Publication: Coleman et al Physiol Genomics. 2003 Feb 6. 12(3):221-8.

Backbone

• Vector backbone: N/A
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Transform into DH5alpha and grow in Luria Broth containing 100µg/ml ampicillin.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: gag, pol, tat, rev
• Species: Retrovirus

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: N/A

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• A portion of this plasmid was derived from a plasmid made by: Original plasmid sent by Jason Coleman and Matthew Huentelman
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid forms part of a plasmid set for lentivirus generation in the Kasparov lab, University of Bristol, UK.

Addgene was unable to obtain quality control sequence for this plasmid. The depositing lab suggested the following diagnostic digest: HindIII digest, expect 0.55, 1.0, and 3.8 kb fragments. Our results for the digest can be seen under "Reviews" on this page.