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Addgene

YEpADE1
(Plasmid #22495)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22495 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    YCplac181
  • Backbone manufacturer
    R. D. Gietz
  • Backbone size w/o insert (bp) 5741
  • Vector type
    yeast shuttle
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ADE1
  • Alt name
    SAICAR synthetase
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1929
  • Mutation
    Original 4067 bp HindIII-HindIII insertion (see YCplacADE1) was cut down using internal EcoRI site and vector EcoRI site
  • GenBank ID
    M61209
  • Entrez Gene
    ADE1 (a.k.a. YAR015W)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    David Kaback

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YEpADE1 was a gift from Beth Montelone (Addgene plasmid # 22495 ; http://n2t.net/addgene:22495 ; RRID:Addgene_22495)
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