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Addgene

pCascade_SPS1(PmcCAST)
(Plasmid #224926)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224926 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDFDuet-1
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Cas5, Cas6, Cas7, Cas8, Cas11

Gene/Insert 2

  • Gene/Insert name
    CRISPR assay for crRNA expression

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCascade_SPS1(PmcCAST) was a gift from Leifu Chang (Addgene plasmid # 224926 ; http://n2t.net/addgene:224926 ; RRID:Addgene_224926)

  • For your References section:

    Biochemical reconstitution of a type I-B CRISPR-associated transposon. Shukun Wang, Leifu Chang. Academic Press, Volume 712, 2025, Pages 55-79, ISSN 0076-6879, ISBN 9780443317521 10.1016/bs.mie.2025.01.042
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