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Addgene

pAcGFP-C1-DGKd2
(Plasmid #224897)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224897 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAcGFP-C1
  • Backbone manufacturer
    Clontech Laboratories, Inc. A Takara Bio Company
  • Backbone size w/o insert (bp) 4731
  • Total vector size (bp) 8373
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DGKD
  • Alt name
    Diacylglycerol kinase delta 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3642
  • GenBank ID
    NM_152879.3
  • Entrez Gene
    DGKD (a.k.a. DGK-delta, DGKdelta, dgkd-2)
  • Promoter CMV
  • Tag / Fusion Protein
    • AcGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcpRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAcGFP-C1-DGKd2 was a gift from Chiaki Murakami (Addgene plasmid # 224897 ; http://n2t.net/addgene:224897 ; RRID:Addgene_224897)
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