pmCherry-C1-PHOSPHO1
(Plasmid #224896)

• Purpose: Express N-terminal mCherry fused to human PHOSPHO1 in mammalian cells
• Depositing Lab: Chiaki Murakami
• Publication: PHOSPHO2 is PA phosphatase and PHOSPHO1 is PLC (unpublished)

Backbone

• Vector backbone: pmCherry-C1
• Backbone manufacturer: Clontech Laboratories, Inc. A Takara Bio Company
• Backbone size w/o insert (bp): 4722
• Total vector size (bp): 5529
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PHOSPHO1
• Alt name: phosphoethanolamine/phosphocholine phosphatase 1
• Species: H. sapiens (human)
• Insert Size (bp): 801
• Mutation: start codon is deleted
• GenBank ID: NM_178500.4
• Entrez Gene: PHOSPHO1
• Entrez Gene: PHOSPHO1
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (N terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CGCAAATGGGCGGTAGGCGTG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry