pEGFP-C1-LgBiT-MYPT1(1-314)(KAKA)-Flag
(Plasmid
#224894)
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PurposeMammalian expression of LgBiT-MYPT1(1--314)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 224894 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLgBiT-MYPT1(1-314)KAKA-Flag
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SpeciesH. sapiens (human)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-C1-LgBiT-MYPT1(1-314)(KAKA)-Flag was a gift from Mathieu Bollen (Addgene plasmid # 224894 ; http://n2t.net/addgene:224894 ; RRID:Addgene_224894) -
For your References section:
A split-luciferase lysate-based approach to identify small-molecule modulators of phosphatase subunit interactions. Claes Z, Bollen M. Cell Chem Biol. 2023 Dec 21;30(12):1666-1679.e6. doi: 10.1016/j.chembiol.2023.07.018. Epub 2023 Aug 24. 10.1016/j.chembiol.2023.07.018 PubMed 37625414