pET16b-MYPT1(1-314)
(Plasmid
#224893)
-
PurposeBacterial expression of MYPT1(1-314)
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 224893 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepET16b
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namemyosin phosphatase targeting subunit 1
-
Alt nameMYPT1
-
Entrez GenePPP1R12A (a.k.a. GUBS, M130, MBS, MYPT1)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pET16b-MYPT1(1-314) was a gift from Mathieu Bollen (Addgene plasmid # 224893 ; http://n2t.net/addgene:224893 ; RRID:Addgene_224893) -
For your References section:
A split-luciferase lysate-based approach to identify small-molecule modulators of phosphatase subunit interactions. Claes Z, Bollen M. Cell Chem Biol. 2023 Dec 21;30(12):1666-1679.e6. doi: 10.1016/j.chembiol.2023.07.018. Epub 2023 Aug 24. 10.1016/j.chembiol.2023.07.018 PubMed 37625414