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Addgene

pRS415 GPDpro-RNQ1-CFP
(Plasmid #224887)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224887 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS415
  • Backbone size w/o insert (bp) 7610
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    RNQ1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1215
  • Entrez Gene
    RNQ1 (a.k.a. YCL028W)
  • Promoter GPD
  • Tag / Fusion Protein
    • CFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XmaI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer M13 REV
  • 3′ sequencing primer M13 FWD
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS415 GPDpro-RNQ1-CFP was a gift from Paul Herman (Addgene plasmid # 224887 ; http://n2t.net/addgene:224887 ; RRID:Addgene_224887)
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