pRS416 CUP1pro-Htt103QdeltaP141-160-YFP
(Plasmid #224886)

• Purpose: Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the middle 20 amino acids of the proline-rich region (141-160) tagged with YFP using copper.
• Depositing Lab: Paul Herman
• Publication: Herman lab plasmids 2024 (unpublished)

Backbone

• Vector backbone: pRS416
• Backbone size w/o insert (bp): 6070
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Htt103QdeltaP141-160
• Alt name: HTT103QdeltaP(Truncation2+AVA)
• Species: H. sapiens (human)
• Insert Size (bp): 432
• Mutation: deltaP141-160
• Entrez Gene: HTT (a.k.a. HD, IT15, LOMARS)
• Promoter: CUP1
• Tag / Fusion Protein:
  • YFP (C terminal on insert)

Cloning Information

• Cloning method: Golden Gate
• 5′ sequencing primer: M13 FWD
• 3′ sequencing primer: M13 REV

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry