pRS416 CUP1pro-Htt103QdeltaP125-160-YFP
(Plasmid
#224883)
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PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct without the first 36 amino acids of the proline-rich region (125-160) tagged with YFP using copper.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 224883 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS416
- Backbone size w/o insert (bp) 6070
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameHtt103QdeltaP125-160
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Alt nameHTT103QdeltaP(REVTruncation3)
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SpeciesH. sapiens (human)
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Insert Size (bp)384
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MutationdeltaP125-160
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Entrez GeneHTT (a.k.a. HD, IT15, LOMARS)
- Promoter CUP1
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Tag
/ Fusion Protein
- YFP (C terminal on insert)
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer M13 FWD
- 3′ sequencing primer M13 REV (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS416 CUP1pro-Htt103QdeltaP125-160-YFP was a gift from Paul Herman (Addgene plasmid # 224883 ; http://n2t.net/addgene:224883 ; RRID:Addgene_224883)