pRS416 GAL1pro-YFP
(Plasmid
#224875)
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Purpose(Empty Backbone) Low copy plasmid with GAL1-driven YFP for C-terminal tagging (no start codon)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 224875 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS416
- Backbone size (bp) 6277
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Vector typeYeast Expression
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Selectable markersURA3
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Tag
/ Fusion Protein
- YFP (C terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- YFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Low copy yeast expression empty vector with GAL1 promoter and YFP for C-terminal tagging. Perfect for cloning an insert with XbaI.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS416 GAL1pro-YFP was a gift from Paul Herman (Addgene plasmid # 224875 ; http://n2t.net/addgene:224875 ; RRID:Addgene_224875)
