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Addgene

pBSsgRNA
(Plasmid #224867)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224867 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript II KS (+)
  • Vector type
    Yeast Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    can also use HB101 (JM110 for cutting by BclI)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The CRISPR/Cas9 system component genes came from pML104 (addgene plasmid #67638, Laughery et. 2015).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

oripUC PSNR52::sgRNA cassette

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBSsgRNA was a gift from Katsunori Suzuki (Addgene plasmid # 224867 ; http://n2t.net/addgene:224867 ; RRID:Addgene_224867)

  • For your References section:

    Conversion of polyploid and alloploid Saccharomyces sensu stricto strains to leu2 mutants by genome DNA editing. Kiyokawa K, Sakuma T, Moriguchi K, Sugiyama M, Akao T, Yamamoto T, Suzuki K. Appl Microbiol Biotechnol. 2024 Jul 12;108(1):416. doi: 10.1007/s00253-024-13242-y. 10.1007/s00253-024-13242-y PubMed 38995331
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