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Addgene

LPUtopia-8
(Plasmid #224781)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224781 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    LPUtopia-7
  • Backbone manufacturer
    Balazsi Lab
  • Backbone size w/o insert (bp) 6823
  • Total vector size (bp) 8916
  • Vector type
    Mammalian Expression, Synthetic Biology
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    puromycin N-acetyltransferase
  • Alt name
    PuroR
  • Species
    Synthetic
  • Insert Size (bp)
    600
  • Promoter CMV

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    enhanced Green Fluorescence Protein
  • Alt name
    EGFP
  • Species
    Synthetic
  • Insert Size (bp)
    719
  • Promoter CMV

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    Red Fluorescence Protein
  • Alt name
    RFP
  • Species
    Synthetic
  • Insert Size (bp)
    699
  • Promoter CMV

Cloning Information for Gene/Insert 3

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There are some discrepancies between Addgene's sequence and the depositor-provided reference, however the depositor has confirmed these changes do not affect plasmid function.

Please visit https://doi.org/10.1101/2024.05.11.593702 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LPUtopia-8 was a gift from Gabor Balazsi (Addgene plasmid # 224781 ; http://n2t.net/addgene:224781 ; RRID:Addgene_224781)

  • For your References section:

    Optimizing a CRISPR-Cas13d Gene Circuit for Tunable Target RNA Downregulation with Minimal Collateral RNA Cutting. Wan Y, Helenek C, Coraci D, Balazsi G. ACS Synth Biol. 2024 Oct 18;13(10):3212-3230. doi: 10.1021/acssynbio.4c00271. Epub 2024 Oct 8. 10.1021/acssynbio.4c00271 PubMed 39377757
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