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pRetroQ-GTSE1-acGFP
(Plasmid #224740)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224740 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRetroQ
  • Backbone manufacturer
    Takara Bio
  • Vector type
    Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GTSE1
  • Alt name
    B99
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2200
  • Entrez Gene
    GTSE1 (a.k.a. B99)
  • Promoter CMV
  • Tag / Fusion Protein
    • acGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer CMV Forward
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.06.26.600797 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRetroQ-GTSE1-acGFP was a gift from Michele Pagano (Addgene plasmid # 224740 ; http://n2t.net/addgene:224740 ; RRID:Addgene_224740)

  • For your References section:

    Stabilization of GTSE1 by cyclin D1-CDK4/6 promotes cell proliferation: relevance in cancer prognosis. García-Vázquez N, González-Robles TJ, Lane E, Spasskaya D, Zhang Q, Kerzhnerman M, Jeong YT, Collu M, Simoneschi D, Ruggles KV, Rona G, Pagano M, Kaisari S. bioRxiv 2024.06.26.600797 10.1101/2024.06.26.600797
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