pcDNA3.1-HA-GTSE1-S734A
(Plasmid
#224736)
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PurposeFor expression of HA-tagged GTSE1 protein with S734A mutation
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 224736 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGTSE1
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Alt nameB99
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SpeciesH. sapiens (human)
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Insert Size (bp)2200
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MutationSerine 734 to Alanine
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Entrez GeneGTSE1 (a.k.a. B99)
- Promoter CMV
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Tag
/ Fusion Protein
- HA (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.06.26.600797 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-HA-GTSE1-S734A was a gift from Michele Pagano (Addgene plasmid # 224736 ; http://n2t.net/addgene:224736 ; RRID:Addgene_224736) -
For your References section:
Stabilization of GTSE1 by cyclin D1-CDK4/6 promotes cell proliferation: relevance in cancer prognosis. García-Vázquez N, González-Robles TJ, Lane E, Spasskaya D, Zhang Q, Kerzhnerman M, Jeong YT, Collu M, Simoneschi D, Ruggles KV, Rona G, Pagano M, Kaisari S. bioRxiv 2024.06.26.600797 10.1101/2024.06.26.600797