pFBOH-MHL.RING1B
(Plasmid
#224712)
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PurposeExpresses N-terminal hexahistidine-tagged human RING1B (RNF2) in insect cells.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 224712 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepFBOH-MHL
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Backbone manufacturerStructural Genomics Consortium
- Backbone size w/o insert (bp) 4734
- Total vector size (bp) 5739
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Vector typeInsect Expression
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Selectable markersGentamicin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRING1b
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Alt nameRNF2; ring finger protein 2; BAP1; DING; BAP-1; HIPI3; RING2; LUSYAM; RING1B
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SpeciesH. sapiens (human)
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Insert Size (bp)1005
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GenBank ID6045
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Entrez GeneRNF2 (a.k.a. BAP-1, BAP1, DING, HIPI3, LUSYAM, RING1B, RING2)
- Promoter Polyhedrin
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Tag
/ Fusion Protein
- 6x histidine tag (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer ttgtatttccagggcTCTCAGGCTGTG CAGACAAAC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFBOH-MHL.RING1B was a gift from Chen Davidovich (Addgene plasmid # 224712 ; http://n2t.net/addgene:224712 ; RRID:Addgene_224712) -
For your References section:
Dynamic PRC1-CBX8 stabilizes a porous structure of chromatin condensates. Uckelmann M, Levina V, Taveneau C, Han Ng X, Pandey V, Martinez J, Mendiratta S, Houx J, Boudes M, Venugopal H, Trepout S, Zhang Q, Flanigan S, Li M, Sierecki E, Gambin Y, Das PP, Bell O, de Marco A, Davidovich C. bioRxiv [Preprint]. 2024 Feb 12:2023.05.08.539931. doi: 10.1101/2023.05.08.539931. 10.1101/2023.05.08.539931 PubMed 38405976