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Addgene

pFBOH-MHL.BMI1
(Plasmid #224711)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224711 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFBOH-MHL
  • Backbone manufacturer
    Structural Genomics Consortium
  • Backbone size w/o insert (bp) 4734
  • Total vector size (bp) 5708
  • Vector type
    Insect Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BMI1
  • Alt name
    proto-oncogene, polycomb ring finger; PCGF4; RNF51; FLVI2/BMI1; flvi-2/bmi-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    975
  • GenBank ID
    648
  • Entrez Gene
    BMI1 (a.k.a. FLVI2/BMI1, PCGF4, RNF51, flvi-2/bmi-1)
  • Promoter Polyhedrin
  • Tag / Fusion Protein
    • 6x histidine tag (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ttgtatttccagggcCATCGAACAACG AGAATCAAG
  • 3′ sequencing primer caagcttcgtcatcaACCAGAAGAAG TTGCTGATGA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFBOH-MHL.BMI1 was a gift from Chen Davidovich (Addgene plasmid # 224711 ; http://n2t.net/addgene:224711 ; RRID:Addgene_224711)

  • For your References section:

    Dynamic PRC1-CBX8 stabilizes a porous structure of chromatin condensates. Uckelmann M, Levina V, Taveneau C, Han Ng X, Pandey V, Martinez J, Mendiratta S, Houx J, Boudes M, Venugopal H, Trepout S, Zhang Q, Flanigan S, Li M, Sierecki E, Gambin Y, Das PP, Bell O, de Marco A, Davidovich C. bioRxiv [Preprint]. 2024 Feb 12:2023.05.08.539931. doi: 10.1101/2023.05.08.539931. 10.1101/2023.05.08.539931 PubMed 38405976
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