pAL113
(Plasmid #22471)

• Purpose: Expresses N-terminal domains of PhyB (with Y276H mutation), fused to mCFP and HRas C terminal CAAX palmitoylation sequence. For red-light-induced binding to PIF6.
• Depositing Lab: Christopher Voigt
• Publication: Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5369
• Vector type: Mammalian Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PhyB NT
• Alt name: phyB
• Species: M. musculus (mouse), A. thaliana (mustard weed)
• Insert Size (bp): 2681
• Mutation: Y276H
• Entrez Gene: PHYB (a.k.a. AT2G18790, HY3, MSF3.17, MSF3_17, OOP1, OUT OF PHASE 1, PHYTOCHROME B, phytochrome B)
• Promoter: CMV
• Tag / Fusion Protein:
  • 10aaLinker-mCFP-HrasCT (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn1 (not destroyed)
• 3′ cloning site: Not1 (not destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry