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pET3a.H2B-K120C
(Plasmid #224706)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224706 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET3a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 4601
  • Total vector size (bp) 4993
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Glucose supplementation advised
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    H2B-K120C
  • Alt name
    Human Histone 2B mutant, H2B K120C mt
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    380
  • Mutation
    K120C
  • Entrez Gene
    H2B
  • Promoter T7

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    With thanks for the wild-type H2B plasmid from David Tremethick, Australian National University.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET3a.H2B-K120C was a gift from Chen Davidovich (Addgene plasmid # 224706 ; http://n2t.net/addgene:224706 ; RRID:Addgene_224706)

  • For your References section:

    Dynamic PRC1-CBX8 stabilizes a porous structure of chromatin condensates. Uckelmann M, Levina V, Taveneau C, Han Ng X, Pandey V, Martinez J, Mendiratta S, Houx J, Boudes M, Venugopal H, Trepout S, Zhang Q, Flanigan S, Li M, Sierecki E, Gambin Y, Das PP, Bell O, de Marco A, Davidovich C. bioRxiv [Preprint]. 2024 Feb 12:2023.05.08.539931. doi: 10.1101/2023.05.08.539931. 10.1101/2023.05.08.539931 PubMed 38405976
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