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Addgene

pBTR1
(Plasmid #22468)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22468 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBCYC1
  • Backbone manufacturer
    PMID 9558351)
  • Backbone size w/o insert (bp) 5279
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cytochrome c
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    321
  • Entrez Gene
    CYCS (a.k.a. CYC, HCS, THC4)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer AGCTCGTTACCCGGGGATC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    W.B. Pollock
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For an explanation of the cytochrome c expression system, see "Bacterial expression of a mitochondrial cytochrome c. Trimethylation of lys72 in yeast iso-1-cytochrome c and the alkaline conformational transition." by Pollock et al. PMID 9558351

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBTR1 was a gift from Gary Pielak (Addgene plasmid # 22468 ; http://n2t.net/addgene:22468 ; RRID:Addgene_22468)

  • For your References section:

    Stability and apoptotic activity of recombinant human cytochrome c. Olteanu A, Patel CN, Dedmon MM, Kennedy S, Linhoff MW, Minder CM, Potts PR, Deshmukh M, Pielak GJ. Biochem Biophys Res Commun. 2003 Dec 19. 312(3):733-40. 10.1016/j.bbrc.2003.10.182 PubMed 14680826
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