p3Eegfp
(Plasmid #22462)

• Depositing Lab: Nathan Lawson
• Publication: Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Backbone

• Vector backbone: pDONRP2r-P3
• Backbone size w/o insert (bp): 3000
• Vector type: Entry Vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EGFP
• Insert Size (bp): 700
• Entrez Gene: eGFP (a.k.a. pPRS3a_01)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13_puc_F
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This clone was amplified with an attB2 site, at the 5’ end and a attB3 site on the 3’ end. The product was then recombined with the pDNR P2R-P3 vector.

Can be used to make 3’ end (C-Term) tagged fusion construct.