pBbA6c_PsulA_sfGFP
(Plasmid
#224587)
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PurposeSOS response reporter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 224587 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep15A
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesfGFP
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SpeciesSynthetic
- Promoter PsulA
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer GGATCTCGACGCTCTCCCTTATGC
- 3′ sequencing primer AAGCGGAATATATCCCTAGGTATAAACGCAGAAAGGCCCACC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBbA6c_PsulA_sfGFP was a gift from Ophelia Venturelli (Addgene plasmid # 224587 ; http://n2t.net/addgene:224587 ; RRID:Addgene_224587) -
For your References section:
Efficient plasmid transfer via natural competence in a microbial co-culture. Cheng YY, Zhou Z, Papadopoulos JM, Zuke JD, Falbel TG, Anantharaman K, Burton BM, Venturelli OS. Mol Syst Biol. 2023 Mar 9;19(3):e11406. doi: 10.15252/msb.202211406. Epub 2023 Jan 30. 10.15252/msb.202211406 PubMed 36714980