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Addgene

pBbA6c_PsulA_sfGFP
(Plasmid #224587)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224587 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    p15A
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sfGFP
  • Species
    Synthetic
  • Promoter PsulA

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GGATCTCGACGCTCTCCCTTATGC
  • 3′ sequencing primer AAGCGGAATATATCCCTAGGTATAAACGCAGAAAGGCCCACC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBbA6c_PsulA_sfGFP was a gift from Ophelia Venturelli (Addgene plasmid # 224587 ; http://n2t.net/addgene:224587 ; RRID:Addgene_224587)
  • For your References section:

    Efficient plasmid transfer via natural competence in a microbial co-culture. Cheng YY, Zhou Z, Papadopoulos JM, Zuke JD, Falbel TG, Anantharaman K, Burton BM, Venturelli OS. Mol Syst Biol. 2023 Mar 9;19(3):e11406. doi: 10.15252/msb.202211406. Epub 2023 Jan 30. 10.15252/msb.202211406 PubMed 36714980