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Addgene

pEZYeGFP-SARS-CoV-2E GGG
(Plasmid #224582)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224582 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEZYeGFP
  • Backbone manufacturer
    pEGFPC4 converted by Yu-Zhu Zhan lab. Addgene 18671
  • Backbone size w/o insert (bp) 7040
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2E GGG
  • Species
    SARS-CoV-2
  • Insert Size (bp)
    228
  • Mutation
    PBM (LLV) mutated to GGG
  • Entrez Gene
    E (a.k.a. GU280_gp04)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEZYeGFP-SARS-CoV-2E GGG was a gift from Isabel Mérida (Addgene plasmid # 224582 ; http://n2t.net/addgene:224582 ; RRID:Addgene_224582)

  • For your References section:

    Identification of Host PDZ-Based Interactions with the SARS-CoV-2 E Protein in Human Monocytes. Avila-Flores A, Sanchez-Cabezon JJ, Ochoa-Echeverria A, Checa AI, Rosas-Garcia J, Tellez-Araiza M, Casado S, Liebana R, Santos-Mendoza T, Merida I. Int J Mol Sci. 2023 Aug 14;24(16):12793. doi: 10.3390/ijms241612793. 10.3390/ijms241612793 PubMed 37628973
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