pEZYeGFP-SARS-CoV-2E stop
(Plasmid
#224581)
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PurposeTo express a GFP tagged version of the Envelope protein of SARS-CoV-2 lacking its PBM in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 224581 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEZYeGFP
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Backbone manufacturerpEGFPC4 converted by Yu-Zhu Zhan lab. Addgene 18671
- Backbone size w/o insert (bp) 7040
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSARS-CoV-2E stop
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SpeciesSARS-CoV-2
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Insert Size (bp)228
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Mutationstop codon before PBM
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Entrez GeneE (a.k.a. GU280_gp04)
- Promoter CMV
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Tag
/ Fusion Protein
- GFP (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEZYeGFP-SARS-CoV-2E stop was a gift from Isabel Mérida (Addgene plasmid # 224581 ; http://n2t.net/addgene:224581 ; RRID:Addgene_224581) -
For your References section:
Identification of Host PDZ-Based Interactions with the SARS-CoV-2 E Protein in Human Monocytes. Avila-Flores A, Sanchez-Cabezon JJ, Ochoa-Echeverria A, Checa AI, Rosas-Garcia J, Tellez-Araiza M, Casado S, Liebana R, Santos-Mendoza T, Merida I. Int J Mol Sci. 2023 Aug 14;24(16):12793. doi: 10.3390/ijms241612793. 10.3390/ijms241612793 PubMed 37628973