pEZYeGFP-SARS-CoV-2E
(Plasmid #224580)

• Purpose: To express a GFP tagged version of the Envelope protein of SARS-CoV-2 in mammalian cells
• Depositing Lab: Isabel Mérida
• Publication: Avila-Flores et al Int J Mol Sci. 2023 Aug 14;24(16):12793. doi: 10.3390/ijms241612793.

Backbone

• Vector backbone: pEZYeGFP
• Backbone manufacturer: pEGFPC4 converted by Yu-Zhu Zhan lab. Addgene 18671
• Backbone size w/o insert (bp): 7040
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SARS-CoV-2E
• Species: SARS-CoV-2
• Insert Size (bp): 228
• Entrez Gene: E (a.k.a. GU280_gp04)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: EGFP-C

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Fritz Roth (Addgene plasmid # 141273)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry