pSuper DGK alfa mouse
(Plasmid
#224573)
-
PurposeNegative Control for expression downregulation of DGK alfa human in human cells
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 224573 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepSUPER
-
Backbone manufacturerOligoengine
- Backbone size w/o insert (bp) 3176
-
Vector typeRNAi
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namesh RNAi Diacylglycerol kinase alfa mouse
-
gRNA/shRNA sequenceACACAAGACCACAGATGATttcaagagaATCATCTGTGGTCTTGTGT
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)64
-
Entrez GeneDgka (a.k.a. 80kDa, AW146112, Dagk1)
- Promoter H1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Bgl2 (destroyed during cloning)
- 3′ cloning site Hind3 (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pSuper DGK alfa mouse was a gift from Isabel Mérida (Addgene plasmid # 224573 ; http://n2t.net/addgene:224573 ; RRID:Addgene_224573) -
For your References section:
Predominant contribution of DGKzeta over DGKalpha in the control of PKC/PDK-1-regulated functions in T cells. Avila-Flores A, Arranz-Nicolas J, Andrada E, Soutar D, Merida I. Immunol Cell Biol. 2017 Jul;95(6):549-563. doi: 10.1038/icb.2017.7. Epub 2017 Feb 6. 10.1038/icb.2017.7 PubMed 28163304