Skip to main content
Addgene

pMXS-IP DEPP1-mCherry
(Plasmid #224559)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224559 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pMXS
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DEPP1
  • Species
    H. sapiens (human)
  • Entrez Gene
    DEPP1 (a.k.a. C10orf10, DEPP, FIG, Fseg)
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer gcttggatacacgccgc
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMXS-IP DEPP1-mCherry was a gift from William Kaelin & Gregory Wyant (Addgene plasmid # 224559 ; http://n2t.net/addgene:224559 ; RRID:Addgene_224559)

  • For your References section:

    Induction of DEPP1 by HIF Mediates Multiple Hallmarks of Ischemic Cardiomyopathy. Wyant GA, Jiang Q, Singh M, Qayyum S, Levrero C, Maron BA, Kaelin WG Jr. Circulation. 2024 Jun 17. doi: 10.1161/CIRCULATIONAHA.123.066628. 10.1161/CIRCULATIONAHA.123.066628 PubMed 38881449
Related items: