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Addgene

pGWB402SN
(Plasmid #224551)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224551 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGWB402N
  • Backbone size (bp) 11331
  • Vector type
    Plant Expression
  • Promoter 2 X 35S
  • Tag / Fusion Protein
    • HA-Twin-Strep (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Unknown

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.07.14.603419 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGWB402SN was a gift from Paul Schulze-Lefert (Addgene plasmid # 224551 ; http://n2t.net/addgene:224551 ; RRID:Addgene_224551)

  • For your References section:

    The barley MLA13-AVRA13 heterodimer reveals principles for immunoreceptor recognition of RNase-like powdery mildew effectors. Lawson AW, Flores-Ibarra A, Cao Y, An C, Neumann U, Gunkel M, Saur IML, Chai J, Behrmann E, Schulze-Lefert P. bioRxiv 2024.07.14.603419 10.1101/2024.07.14.603419
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