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pME nls BFP FLAG WPRE bGH pA (JDW 484)
(Plasmid #224539)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224539 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR221
  • Vector type
    Gateway cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nls-mTagBFP2-FLAG_WPRE-bGH-pA
  • Species
    Synthetic

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME nls BFP FLAG WPRE bGH pA (JDW 484) was a gift from Joshua Wythe (Addgene plasmid # 224539 ; http://n2t.net/addgene:224539 ; RRID:Addgene_224539)

  • For your References section:

    Multisite Assembly of Gateway Induced Clones (MAGIC): a flexible cloning toolbox with diverse applications in vertebrate model systems. Gillespie W, Zhang Y, Ruiz OE, Cerda III J, Ortiz-Guzman J, Turner WD, Largoza G, Sherman M, Mosser LE, Fujimoto E, Chien C-B, Kwan KM, Arenkiel BR, Devine WP, Wythe JD. bioRxiv 2024.07.13.603267 10.1101/2024.07.13.603267
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