p3E-EF1a-pA (JDW 1222)
(Plasmid
#224526)
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PurposeA Gateway compatible 3' entry clone containing rat EF-1a polyA
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 224526 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR P2R P3
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Vector typeGateway cloning
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEF-1a polyA
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SpeciesR. norvegicus (rat)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p3E-EF1a-pA (JDW 1222) was a gift from Joshua Wythe (Addgene plasmid # 224526 ; http://n2t.net/addgene:224526 ; RRID:Addgene_224526) -
For your References section:
Multisite Assembly of Gateway Induced Clones (MAGIC): a flexible cloning toolbox with diverse applications in vertebrate model systems. Gillespie W, Zhang Y, Ruiz OE, Cerda III J, Ortiz-Guzman J, Turner WD, Largoza G, Sherman M, Mosser LE, Fujimoto E, Chien C-B, Kwan KM, Arenkiel BR, Devine WP, Wythe JD. bioRxiv 2024.07.13.603267 10.1101/2024.07.13.603267