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pLUEF1 Luc2-IRES-GFP
(Plasmid #224467)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224467 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLUEF1-IRES-GFP
  • Modifications to backbone
    Luc2 was amplified from pFU Luc2-eGFP (a gift from Professor Jennifer Prescher) plasmid using primers: cgagactagcctcgaggtttaaacATGGAAGATGCCAAAAAC forward and attcctgcagcccgtagtttaaacTTATTACACGGCGATCTTG reverse and Gibson cloned into pLUEF1-IRES-GFP between Pmei restriction sites.
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Luciferase
  • Alt name
    Luc2
  • Species
    Photonis pyralis (firefly)
  • Insert Size (bp)
    1656
  • GenBank ID
    AY738222 AY738222
  • Promoter EF1a

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TCAAGCCTCAGACAGTGGTTC
  • 3′ sequencing primer CCTCACATTGCCAAAAGACG
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The Luc2 gene was amplified from pFU Luc2-eGFP (a gift from Professor Jennifer Prescher)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLUEF1 Luc2-IRES-GFP was a gift from Robert Kerbel (Addgene plasmid # 224467 ; http://n2t.net/addgene:224467 ; RRID:Addgene_224467)

  • For your References section:

    Immunological Tolerance to Luciferase and Fluorescent Proteins using Tol Mice Enables Development of Improved Tumor Models for Investigating Immunity and Metastasis. Khan KA, Qureshi AA, Xu P, Kuo HY, Schumacher TN, Michael IP, Kerbel RS. Cancer Res. 2025 Apr 14. doi: 10.1158/0008-5472.CAN-24-2418. 10.1158/0008-5472.CAN-24-2418 PubMed 40228139
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