Skip to main content
Addgene

pLUEF1 IRES GFP (backbone)
(Plasmid #224466)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224466 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pWPi
  • Modifications to backbone
    The minimal ubiquitous chromatin opening element (UCOE) from human CBX3 promoter was amplified from pMH0006 plasmid (a gift from Martin Kampmann & Jonathan Weissman (Addgene plasmid # 135448; RRID:Addgene_135448)) using the following primers aagaccaccgcacagcaagcgcggccgcTGATCTTCAGACCTGGAGG forward and caggcaccagagcaggccggggccggccAGCTTGAGACTACCCCCG reverse and then Gibson cloned (Gibson Assembly Mastermix, New England Biolabs # E2611S) into pWPi (a gift from Didier Trono (Addgene plasmid # 12254; RRID:Addgene_12254) between restriction sites Noti and Fsei to create pLUEF1 IRES GFP lentiviral vector.
  • Vector type
    Lentiviral
  • Promoter Ef1a

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLUEF1 IRES GFP (backbone) was a gift from Robert Kerbel (Addgene plasmid # 224466 ; http://n2t.net/addgene:224466 ; RRID:Addgene_224466)

  • For your References section:

    Immunological Tolerance to Luciferase and Fluorescent Proteins using Tol Mice Enables Development of Improved Tumor Models for Investigating Immunity and Metastasis. Khan KA, Qureshi AA, Xu P, Kuo HY, Schumacher TN, Michael IP, Kerbel RS. Cancer Res. 2025 Apr 14. doi: 10.1158/0008-5472.CAN-24-2418. 10.1158/0008-5472.CAN-24-2418 PubMed 40228139
Related items: