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Mito-CanlonicSF/pCMV-myc-mito
(Plasmid #224465)

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Item Catalog # Description Quantity Price (USD)
Plasmid 224465 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pCMV-myc-Mito
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4972
  • Total vector size (bp) 6730
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CanlonicSF
  • Alt name
    Mitocondrial Calcium-and-Lactate Optical Nano-Indicator from CECs Single Fluorophore
  • Species
    Synthetic
  • Insert Size (bp)
    1757
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer BGH Reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.07.19.604361 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mito-CanlonicSF/pCMV-myc-mito was a gift from Alejandro San Martín (Addgene plasmid # 224465 ; http://n2t.net/addgene:224465 ; RRID:Addgene_224465)

  • For your References section:

    Lactate-carried Mitochondrial Energy Overflow. Rauseo D, Contreras-Baeza Y, Faurand H, Cárcamo N, Suárez R, von Faber-Castell A, Silva F, Mora-González V, Wyss MT, Baeza-Lehnert F, Ruminot I, Alvarez-Navarro C, Martín AS, Weber B, Sandoval PY, Barros LF. bioRxiv 2024.07.19.604361 10.1101/2024.07.19.604361
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