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Addgene

pLIC-EcTOP
(Plasmid #224463)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224463 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLIC-HK
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Turbo
  • Growth instructions
    1% glucose
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Escherichia coli TopA
  • Alt name
    Escherichia coli topoisomerase I
  • Species
    Escherichia coli
  • Insert Size (bp)
    2595
  • GenBank ID
    X04475.1
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis tag (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLIC-EcTOP was a gift from Yuk-Ching Tse-Dinh (Addgene plasmid # 224463 ; http://n2t.net/addgene:224463 ; RRID:Addgene_224463)

  • For your References section:

    Structural basis for suppression of hypernegative DNA supercoiling by E. coli topoisomerase I. Tan K, Zhou Q, Cheng B, Zhang Z, Joachimiak A, Tse-Dinh YC. Nucleic Acids Res. 2015 Dec 15;43(22):11031-46. doi: 10.1093/nar/gkv1073. Epub 2015 Oct 20. 10.1093/nar/gkv1073 PubMed 26490962
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