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kiCAP-AAV-MyoAAV4E
(Plasmid #224452)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224452 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57-Kan
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
  • Alt name
    MyoAAV 4E
  • Species
    Synthetic
  • Insert Size (bp)
    2232
  • Mutation
    RGDFNNT insert between amino acids 588 and 589 of AAV9 VP1, S586E, A587N, Q588R
  • Promoter p41

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert was cloned via BsiWI (5') and AgeI (3', preserved in cloning) sites

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    kiCAP-AAV-MyoAAV4E was a gift from Pardis Sabeti (Addgene plasmid # 224452 ; http://n2t.net/addgene:224452 ; RRID:Addgene_224452)

  • For your References section:

    Directed evolution of a family of AAV capsid variants enabling potent muscle-directed gene delivery across species. Tabebordbar M, Lagerborg KA, Stanton A, King EM, Ye S, Tellez L, Krunnfusz A, Tavakoli S, Widrick JJ, Messemer KA, Troiano EC, Moghadaszadeh B, Peacker BL, Leacock KA, Horwitz N, Beggs AH, Wagers AJ, Sabeti PC. Cell. 2021 Sep 16;184(19):4919-4938.e22. doi: 10.1016/j.cell.2021.08.028. Epub 2021 Sep 9. 10.1016/j.cell.2021.08.028 PubMed 34506722
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