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Addgene

pET28a-AzaD
(Plasmid #224425)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224425 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 8567
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    azaD
  • Species
    Glycomyces harbinensis ATCC 43155
  • Tag / Fusion Protein
    • His6 (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-AzaD was a gift from Emily Balskus (Addgene plasmid # 224425 ; http://n2t.net/addgene:224425 ; RRID:Addgene_224425)

  • For your References section:

    Discovery of the Azaserine Biosynthetic Pathway Uncovers a Biological Route for alpha-Diazoester Production. Van Cura D, Ng TL, Huang J, Hager H, Hartwig JF, Keasling JD, Balskus EP. Angew Chem Int Ed Engl. 2023 Jul 10;62(28):e202304646. doi: 10.1002/anie.202304646. Epub 2023 May 31. 10.1002/anie.202304646 PubMed 37151182
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