pBAD02_CBASS_Escherichia_coli_K71-77
(Plasmid
#224398)
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PurposeFor expression of the type I CBASS system encoded by Escherichia coli K71-77
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 224398 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBAD02
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePhospholipase_EcCdnE_DisA-like
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SpeciesEscherichia coli K71-77
Cloning Information
- Cloning method Gene Synthesis
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Genome sourced from Integrated Microbial Genomes & Microbiomes (IMG/M), Genome 2663762841
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBAD02_CBASS_Escherichia_coli_K71-77 was a gift from Philip Kranzusch (Addgene plasmid # 224398 ; http://n2t.net/addgene:224398 ; RRID:Addgene_224398) -
For your References section:
A large-scale type I CBASS antiphage screen identifies the phage prohead protease as a key determinant of immune activation and evasion. Richmond-Buccola D, Hobbs SJ, Garcia JM, Toyoda H, Gao J, Shao S, Lee ASY, Kranzusch PJ. Cell Host Microbe. 2024 Jul 10;32(7):1074-1088.e5. doi: 10.1016/j.chom.2024.05.021. Epub 2024 Jun 24. 10.1016/j.chom.2024.05.021 PubMed 38917809