pLenti_CMV_Puro_SFB_GNB1L_WD 6 deletion
(Plasmid
#224387)
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PurposeLenti plasmid for generating GNB1L _WD 6 domain depleted expressing stable cell lines
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 224387 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti-CMV-Puro
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Vector typeMammalian Expression, Bacterial Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGNB1L
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SpeciesH. sapiens (human)
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MutationWD 6 domain deleted (aa 242-282)
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Entrez GeneGNB1L (a.k.a. DGCRK3, FKSG1, GY2, WDR14, WDVCF)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti_CMV_Puro_SFB_GNB1L_WD 6 deletion was a gift from Junjie Chen (Addgene plasmid # 224387 ; http://n2t.net/addgene:224387 ; RRID:Addgene_224387) -
For your References section:
FACS-based genome-wide CRISPR screens define key regulators of DNA damage signaling pathways. Huang M, Yao F, Nie L, Wang C, Su D, Zhang H, Li S, Tang M, Feng X, Yu B, Chen Z, Wang S, Yin L, Mou L, Hart T, Chen J. Mol Cell. 2023 Aug 3;83(15):2810-2828.e6. doi: 10.1016/j.molcel.2023.07.004. 10.1016/j.molcel.2023.07.004 PubMed 37541219