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Addgene

pUC19_GNB1L_dTAG
(Plasmid #224378)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224378 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Bacterial Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GNB1L
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2552
  • Mutation
    Synonymous mutation
  • Entrez Gene
    GNB1L (a.k.a. DGCRK3, FKSG1, GY2, WDR14, WDVCF)
  • Tag / Fusion Protein
    • dTAG (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC19_GNB1L_dTAG was a gift from Junjie Chen (Addgene plasmid # 224378 ; http://n2t.net/addgene:224378 ; RRID:Addgene_224378)

  • For your References section:

    FACS-based genome-wide CRISPR screens define key regulators of DNA damage signaling pathways. Huang M, Yao F, Nie L, Wang C, Su D, Zhang H, Li S, Tang M, Feng X, Yu B, Chen Z, Wang S, Yin L, Mou L, Hart T, Chen J. Mol Cell. 2023 Aug 3;83(15):2810-2828.e6. doi: 10.1016/j.molcel.2023.07.004. 10.1016/j.molcel.2023.07.004 PubMed 37541219
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