Skip to main content
Addgene

pX459_GNB1L_gRNA
(Plasmid #224377)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224377 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    PX459
  • Vector type
    Mammalian Expression, Bacterial Expression, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GNB1L
  • gRNA/shRNA sequence
    AGGATCAGCGGATCAGCCTC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    20
  • Entrez Gene
    GNB1L (a.k.a. DGCRK3, FKSG1, GY2, WDR14, WDVCF)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX459_GNB1L_gRNA was a gift from Junjie Chen (Addgene plasmid # 224377 ; http://n2t.net/addgene:224377 ; RRID:Addgene_224377)

  • For your References section:

    FACS-based genome-wide CRISPR screens define key regulators of DNA damage signaling pathways. Huang M, Yao F, Nie L, Wang C, Su D, Zhang H, Li S, Tang M, Feng X, Yu B, Chen Z, Wang S, Yin L, Mou L, Hart T, Chen J. Mol Cell. 2023 Aug 3;83(15):2810-2828.e6. doi: 10.1016/j.molcel.2023.07.004. 10.1016/j.molcel.2023.07.004 PubMed 37541219
Related items: