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Addgene

PT7lacO-EF-Tu-His8
(Plasmid #224370)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224370 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EF-Tu
  • Species
    E. coli
  • Entrez Gene
    tufB (a.k.a. b3980, ECK3971, kirT, pulT)
  • Tag / Fusion Protein
    • His8 (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

It is best to grow cells in strains with LacIq and in media supplemented 1% glucose for genetic stability. Please visit https://doi.org/10.1101/2024.06.19.599772 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PT7lacO-EF-Tu-His8 was a gift from Richard Murray (Addgene plasmid # 224370 ; http://n2t.net/addgene:224370 ; RRID:Addgene_224370)

  • For your References section:

    Optimizing protein expression in the One-Pot PURE System: Insights into reaction composition and translation efficiency. Zhang Y, Qiu Y, Deveikis M, Martinez ZA, Chou T, Freemont PS, Murray RS. bioRxiv 2024.06.19.599772 10.1101/2024.06.19.599772
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