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pAAV uN2C GFP (Ctl)
(Plasmid #224355)

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Item Catalog # Description Quantity Price (USD)
Plasmid 224355 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pAAV
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    uN2C
  • Alt name
    upstream ORF of the NOTCH2NLC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    168
  • Mutation
    Codon-optimized for expression in human, so no pure GGC repeats (but still encodes for glycine)
  • Promoter CMV + chimeric intyron (CAG)
  • Tag / Fusion Protein
    • eGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HIND3 (not destroyed)
  • 3′ cloning site BAMH1 (not destroyed)
  • 5′ sequencing primer CAG promoter
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV uN2C GFP (Ctl) was a gift from Nicolas Charlet-Berguerand (Addgene plasmid # 224355 ; http://n2t.net/addgene:224355 ; RRID:Addgene_224355)

  • For your References section:

    Translation of GGC repeat expansions into a toxic polyglycine protein in NIID defines a novel class of human genetic disorders: The polyG diseases. Boivin M, Deng J, Pfister V, Grandgirard E, Oulad-Abdelghani M, Morlet B, Ruffenach F, Negroni L, Koebel P, Jacob H, Riet F, Dijkstra AA, McFadden K, Clayton WA, Hong D, Miyahara H, Iwasaki Y, Sone J, Wang Z, Charlet-Berguerand N. Neuron. 2021 Jun 2;109(11):1825-1835.e5. doi: 10.1016/j.neuron.2021.03.038. Epub 2021 Apr 21. 10.1016/j.neuron.2021.03.038 PubMed 33887199
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