pAAV uN2C GFP (Ctl)
(Plasmid #224355)

• Purpose: Express GFP tagged upsteram ORF of NOTCH2NLC with a control size (12x) of GGC repeats
• Depositing Lab: Nicolas Charlet-Berguerand
• Publication: Boivin et al Neuron. 2021 Jun 2;109(11):1825-1835.e5. doi: 10.1016/j.neuron.2021.03.038. Epub 2021 Apr 21.

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Backbone

• Vector backbone: pAAV
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: uN2C
• Alt name: upstream ORF of the NOTCH2NLC
• Species: H. sapiens (human)
• Insert Size (bp): 168
• Mutation: Codon-optimized for expression in human, so no pure GGC repeats (but still encodes for glycine)
• Promoter: CMV + chimeric intyron (CAG)
• Tag / Fusion Protein:
  • eGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HIND3 (not destroyed)
• 3′ cloning site: BAMH1 (not destroyed)
• 5′ sequencing primer: CAG promoter

Resource Information

• Supplemental Documents:
  • pAAV uN2C op GFP.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA