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Addgene

pJExpress401-T4 Lysozyme C54T C97A (WT*)
(Plasmid #224353)

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Item Catalog # Description Quantity Price (USD)
Plasmid 224353 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pJExpress401
  • Backbone manufacturer
    DNA 2.0
  • Backbone size w/o insert (bp) 3936
  • Total vector size (bp) 4461
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    T4 Lysozyme
  • Species
    Bacteriophage T4
  • Insert Size (bp)
    525
  • Mutation
    C45T, C94A
  • GenBank ID
    NP_049736
  • Promoter T5
  • Tag / Fusion Protein
    • TEV-cleavable His6 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CTCGAAAATAATAAAGGGAAAATCAG
  • 3′ sequencing primer TGGTAGTGTGGGGACTC
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Brian Matthews: Addgene 18111 (pHS1403 T4 Lysozyme WT*)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJExpress401-T4 Lysozyme C54T C97A (WT*) was a gift from Terry Watt (Addgene plasmid # 224353 ; http://n2t.net/addgene:224353 ; RRID:Addgene_224353)

  • For your References section:

    An improved 96-well turbidity assay for T4 lysozyme activity. Toro TB, Nguyen TP, Watt TJ. MethodsX. 2015 May 18;2:256-62. doi: 10.1016/j.mex.2015.05.004. eCollection 2015. 10.1016/j.mex.2015.05.004 PubMed 26150996
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