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Addgene

pJExpress401-KDAC8 D101A
(Plasmid #224347)

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Item Catalog # Description Quantity Price (USD)
Plasmid 224347 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pJExpress401
  • Backbone manufacturer
    DNA 2.0
  • Backbone size w/o insert (bp) 3936
  • Total vector size (bp) 5109
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KDAC8
  • Alt name
    HDAC8
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1173
  • Mutation
    Aspartate 101 mutated to alanine
  • Entrez Gene
    HDAC8 (a.k.a. CDA07, CDLS5, HD8, HDACL1, KDAC8, MRXS6, RPD3, WTS)
  • Promoter T5
  • Tag / Fusion Protein
    • TEV-cleavable His6 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CTCGAAAATAATAAAGGGAAAATCAG
  • 3′ sequencing primer TGGTAGTGTGGGGACTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Codon optimized for bacterial expression. The resulting protein has reduced catalytic activity compared to wild-type.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJExpress401-KDAC8 D101A was a gift from Terry Watt (Addgene plasmid # 224347 ; http://n2t.net/addgene:224347 ; RRID:Addgene_224347)

  • For your References section:

    Lysine Deacetylase Substrate Selectivity: A Dynamic Ionic Interaction Specific to KDAC8. Toro TB, Swanier JS, Bezue JA, Broussard CG, Watt TJ. Biochemistry. 2021 Aug 24;60(33):2524-2536. doi: 10.1021/acs.biochem.1c00384. Epub 2021 Aug 6. 10.1021/acs.biochem.1c00384 PubMed 34357750
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