pFastBacI-KDAC6
(Plasmid
#224346)
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PurposeExpresses human KDAC6 (HDAC6) in insect cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 224346 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepFastBacI
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Backbone manufacturerThermo Fisher Scientific
- Backbone size w/o insert (bp) 4682
- Total vector size (bp) 8369
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Modifications to backboneRemoval of one NheI site
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKDAC6
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Alt nameHDAC6
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SpeciesH. sapiens (human)
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Insert Size (bp)3687
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Entrez GeneHDAC6 (a.k.a. CPBHM, HD6, JM21, KDAC6, PPP1R90)
- Promoter Polyhedrin
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Tag
/ Fusion Protein
- TEV-cleavable His6 (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CTAGTGGTTGGCTACGTATACTC
- 3′ sequencing primer CCTCTACAAATGTGGTATGGCTG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byEric Verdin: Addgene 13823 (pcDNA3.1+ HDAC6 Flag).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFastBacI-KDAC6 was a gift from Terry Watt (Addgene plasmid # 224346 ; http://n2t.net/addgene:224346 ; RRID:Addgene_224346) -
For your References section:
Purification of metal-dependent lysine deacetylases with consistently high activity. Toro TB, Painter RG, Haynes RA, Glotser EY, Bratton MR, Bryant JR, Nichols KA, Matthew-Onabanjo AN, Matthew AN, Bratcher DR, Perry CD, Watt TJ. Protein Expr Purif. 2018 Jan;141:1-6. doi: 10.1016/j.pep.2017.08.009. Epub 2017 Aug 24. 10.1016/j.pep.2017.08.009 PubMed 28843507